解放军医学杂志2001，Vol.26Issue(2)：115-116,2.
大鼠C6脑胶质瘤模型的实验研究
THE EXPERIMENTAL STUDY OF A RAT C6 BRAIN-TUMOR MODEL
吴景文 1章翔 1高大宽 1易林华 1李侠 1刘先珍1
作者信息
摘要
Abstract
Rat C6 glioma cells were cultured in vitro，and 10μL C6 cell suspension containing 10g/L agarose and 1×106 C6 cells was injected into the right caudate nucleus of rat brains to establish a rat brain-tumor model by directional implantation method.General observation and MRI scan were conducted after implantation.Trans-aorta paraformaldehyde perfusion for 5 group rats having received implantation was carried out at 10,15,20,25,days and before natural death.The tumor-containing samples were prepared histologically by hematoxylin and eosin stains.MRI scan showed that intracerebral growth occurred in 50 implanted rats,with a distant metastasis of 4%.The results indicated that tumor globular intracerebral growth occurred and extracranial growth extension was scarce after implantation.The model was very stable and the implanted rat survival duration is easily determined. The experiment lays the foundation for chemotherapy,radiotherapy and gene therapy of the glioma.关键词
C6胶质瘤细胞/定向移植/大鼠/脑分类
医药卫生引用本文复制引用
吴景文,章翔,高大宽,易林华,李侠,刘先珍..大鼠C6脑胶质瘤模型的实验研究[J].解放军医学杂志,2001,26(2):115-116,2.
