中国免疫学杂志2001，Vol.17Issue(3)：115-118,4.
IL-6在人骨髓瘤细胞U266中的信号转导途径与细胞增殖促进效应之间的关系
Relationship between IL-6 signal transduction pathways and its growth effect on a human myeloma cell line-U266
摘要
Abstract
Objective:To investigate the relationship between IL-6 signaltransduction pathways and i ts growth effect on a human myeloma cell line-U266.Methods:Effect of IL-6 on the growth of U266 cells was sh own by MTT;Electrophoretic mobility shift assay(EMSA) and immunoprecipitation(IP ) were used to detect the activation of two IL-6 signal transduction pathways.T hen U266 cells were treated with chemical agents of transfected with the expres sion plasmids of the transcription factors,which can up- or down-regulate the activation of o ne of the two pathways specifically.The changes of the proliferation of U266 cel l s and the activation of two signal transduction pathways by IL-6 were also exhi b ited by MTT and EMSA,respectively.Results:1.IL-6 can prom ote the proliferation of U266 cells and activate JAK/STAT and Ras/NF-IL-6 path ways at different dosage.2.Specific up- or down-regulation of Ras/NF-IL-6 pa t hway can argument or weaken the effect of IL-6 on the proliferation of U266 cel ls,but the inhibition of JAK/STAT pathway activation can strengthen the IL-6 ef fect on the cells.Conclusion:There is an opposite relationship between the a ctivation of Ras/NF-IL-6 and JAK/STAT pathways with the effect of IL-6 on the proliferation of U266 cells.关键词
IL-6 骨髓瘤 信号转导 生物学效应分类
医药卫生引用本文复制引用
宋伦,黎燕,沈倍奋..IL-6在人骨髓瘤细胞U266中的信号转导途径与细胞增殖促进效应之间的关系[J].中国免疫学杂志,2001,17(3):115-118,4.基金项目
国家杰出青年科学基金(No39925019) （No39925019）
