中国现代医学杂志2008，Vol.18Issue(9)：1153-1157,1161,6.
肾癌细胞中环氧合酶-2的高表达及NS398对肾癌细胞生长抑制和凋亡作用机制的研究
Enhanced expression of cyclooxygenase (COX)-2 in human renal cell carcinoma cells: evidence for anti-proliferation and apoptosis induction through inhibition of COX-2 expression by NS398
摘要
Abstract
Objective To evaluate the effects of NS398, a selective cyclooxygenase-2 (COX-2) inhibitor on cellular proliferation and apoptosis in human RCC cell line, and to examine possible mechanisms responsible for the anti-proliferative effects of NS398. Methods Human RCC cell line 786-0 was maintained in culture using standard technique and treated with NS398 at doses of 25, 50, 100, 150 and 200μM, respectively. Cellular inhibition was measured by MTT at 24 and 48h. Apoptosis was evaluated by Flow Cytometry. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to examine the expression of COX-2 and Bcl-2 at mRNA and protein levels. Results NS398 exhibited significant cell growth inhibition at 24hr and 48hr in the 786-0 cells in time and concentration-dependent manner. COX-2 and Bcl-2 were significantly decreased by NS398 both at mRNA and protein levels. NS398 inhibited proliferation of 786-0 cells through G0/G1 phase arrest along with induction of apoptosis in dose-dependent manner. Conclusions Over-expression of COX-2 mRNA and protein exist in human 786-0 RCC cell line and COX-2 may play a crucial role in carcinogenesis of RCC. NS398 can suppress the proliferation of 786-0 RCC cells by induction of apoptosis. The possible mechanism of induction of apoptosis in 786-0 cells is inhibition of COX-2 expression and suppression of Bcl-2 by NS398. The results suggest that COX-2 may become a new target gene for RCC treatment, and NS398 may be a potentially effective agent against human RCC expressing COX-2.关键词
肾细胞癌/NS398/COX-2/Bcl-2/凋亡Key words
renal cell carcinoma/NS398/COX-2/Bcl-2/apoptosis分类
医药卫生引用本文复制引用
毕建斌,刘涛,孔垂泽,孙志熙,王侠,李泽良,杨绍波..肾癌细胞中环氧合酶-2的高表达及NS398对肾癌细胞生长抑制和凋亡作用机制的研究[J].中国现代医学杂志,2008,18(9):1153-1157,1161,6.基金项目
Supported by Scientific Research Project for Institute of Higher Education of Education Bureau in Liaoning Province (No.: 202013145) （No.: 202013145）
