中华医学杂志（英文版）2009，Vol.122Issue(10)：1167-1172,6.DOI:10.3760/cma.j.issn.0366-6999.2009.10.010
Screening for genes associated with ovarian cancer prognosis
Screening for genes associated with ovarian cancer prognosis
CHANG Xiao-hong 1ZHANG Li 2YANG Rong 1FENG Jie 1CHENG Ye-xia 1CHENG Hong-yan 1YE Xue 1FU Tian-yun 1CUI Heng1
作者信息
- 1. Gynecologic Oncology Center, Peking University People's Hospital, Beijing 100044, China
- 2. Department of Obstetrics, Shangdi Hospital, Beijing 100084,China
- 折叠
摘要
Abstract
Background Human epithelial ovarian cancer cell line SKOV3.ipl is more invasive and metastatic compared with its parental line SKOV3. A total of 17 000 human genome complementary DNA microarrays were used to compare the gene expression patterns of the two cell lines. Based on this, the gene expression profiles of 22 patients with ovarian cancer were analyzed by cDNA microarray, and screened the 2-fold differentially expressed genes compared with the normal ones. We screened genes relevant to clinical prognosis of serous ovarian cancer by determining the expression profiles of ovarian cancer genes to investigate cell receptor and immunity-associated genes, and as groundwork, identify ovarian cancer-associated antigens at the gene level.Methods Total RNA was extracted from 22 patients with ovarian cancer and DNA microarrays were prepared. After scanning, hybridization signals were collected and the genes that were differentially expressed twice as compared with the normal ones were screened.Results We screened 236 genes relevant to the prognosis of ovarian cancer from the 17 000 human genome cDNA microarrays. According to gene classification, 48 of the 236 genes were cell receptor or immunity-associatad genes,including 2 genes related to the International Federation of Gynecology and Obstetrics (FIGO) stage, 4 genes to histological grade, 18 genes to lymph node metastasis, 11 genes to residual disease, and 13 genes to the reactivity to chemotherapy. Several functionally important genes including fibronectin 1, pericentriolar material 1, beta-2-microglobulin,PPAR binding protein were identified through review of the literature.Conclusions The cDNA microarray of ovarian cancer genes developed in this study was effective and high throughput in screening the ovarian cancer-associated genes differentially expressed. Through the studies of the cell receptor and immunity-associated genes we expect to identify the molecular biology index of ovarian cancer-associated antigens.关键词
ovarian cancer/microarray/cell receptor/immunity-associated/prognosisKey words
ovarian cancer/microarray/cell receptor/immunity-associated/prognosis分类
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CHANG Xiao-hong,ZHANG Li,YANG Rong,FENG Jie,CHENG Ye-xia,CHENG Hong-yan,YE Xue,FU Tian-yun,CUI Heng..Screening for genes associated with ovarian cancer prognosis[J].中华医学杂志（英文版）,2009,122(10):1167-1172,6.
