首页|期刊导航|中华医学杂志（英文版）|Enhancing alkylating agent resistance through ERCC2 gene transfection in human glioma cell line
中华医学杂志（英文版）2003，Vol.116Issue(8)：1171-1174,4.
Enhancing alkylating agent resistance through ERCC2 gene transfection in human glioma cell line
Enhancing alkylating agent resistance through ERCC2 gene transfection in human glioma cell line
摘要
Abstract
Objective To confirm the enhancing effect of excision repair cross complementing rodent repair deficiency gene 2 (ERCC2) on alkylating agents resistance. Methods The authors constructed a pcDNA3-ERCC2 plasmid. The pcDNA3-ERCC2 was transfected into a selected ERCC2 negative human glioma cell line, SKMG-4, using liposome-mediated transfection. After G418 selection, a stable transfected cell line was obtained and tested for cytotoxicity of several alkylating agents. Results The stable transfectant was obtained and confirmed by RT-PCR as well as Western blot analysis to be strongly expressing ERCC2 at both mRNA and protein levels. The IC90 (μmol/L) of two alkylating agents, cisplatin and melphalan, increased from 1.0 to 1.75 (75%) and 5.6 to 9.0 (61%), respectively, compared with control cell line. Conclusion The present data provided evidences and confirmed the authors' previous results that ERCC2 contributes, at least partially, to alkylating agent resistance in human glioma cell line.关键词
drug resistance/ERCC2/nucleotide excision repairKey words
drug resistance/ERCC2/nucleotide excision repair分类
医药卫生引用本文复制引用
CHEN Zhongping 陈忠平,ZHANG Junying 张俊英,Gérard MOHR..Enhancing alkylating agent resistance through ERCC2 gene transfection in human glioma cell line[J].中华医学杂志（英文版）,2003,116(8):1171-1174,4.基金项目
This work was supported by Stating Fundation from Cancer Center, Sun Yat-Sen University of Medical Sciences (NO. 433), China Education Ministry Fundation (2000) and CMB-SUMS Scholar-ship (98-677) （NO. 433）
