中国癌症防治杂志2009，Vol.1Issue(2)：117-120,4.
光动力对人子宫颈癌Hela细胞的杀伤作用及光动力剂量的研究
The effect and dosage of photodynamic therapy on human cervical carcinoma cell line Hela
摘要
Abstract
Objective To observe the effect of 5-Aminolevulinic acid (ALA) mediated photodynamic therapy (PDT) on human cervical carcinoma cell line Hela,and to investigate the best dosage of PDT.Methods Hela cells were divided into four groups:normal control group,ALA group,PDT group and ALA-PDT group.The cells were treated with different concentration of ALA and/or different dose of PDT.MTT assay was applied to detect the inhibition effect of the treatments on Hela cells.The curves of inhibiting rate were draw and the morphological changes on the cells were observed.Results ALA-PDT could inhibit obviously the growth of Hela cells,while there was no obvious effect on cell growth with other treatments.After treating the cells with ALA-PDT for 12 hours,the sign of cell necrosis was obvious and there were a small number of apoptotic cells.Conclusion s The growth of Hela cells is obviously inhibited by ALA-PDT.0.5 mmol/L of ALA and 5J/cm2 of PDT are the best dosages to kill or damage Hela cells.关键词
子宫颈癌/氨基酮戊酸/光动力疗法Key words
Cervical carcinoma/5-Aminolevulinic acid/Photodynamic therapy分类
医药卫生引用本文复制引用
陈闯,陈蔚,黎汉忠,张作军,谭志强..光动力对人子宫颈癌Hela细胞的杀伤作用及光动力剂量的研究[J].中国癌症防治杂志,2009,1(2):117-120,4.基金项目
广西壮族自治区卫生厅自筹经费课题(Z 2005149) （Z 2005149）
