水产学报2008，Vol.32Issue(1)：117-124,8.
尼罗罗非鱼鱼皮胶原蛋白中游离基清除肽的制备及其抗氧化活性
Preparation of radical scavenging peptides from Oreochromis niloticus skin gelatin and its antioxidative activity
摘要
Abstract
Gelatin extracted from tilapia (Oreochromis niloticus) skin was hydrolyzed with serial digestions in the order of Bromelain and Alcalase.The antioxidative activity in vitro of tilapia skin gelatin hydrolysates (TGH) was researched.The results showed TGH had strong superoxide/hydroxyl radical scavenging ability and the IC50 value was 3.13 mg·mL-1 and 3.58 mg·mL-1,respectively.The TGH was fractionated into five major types of TGH-Ⅰ(＞10 ku),TGH-Ⅱ(10-5 ku),TGH-Ⅲ(5-3 ku),TGH-Ⅳ(3-1 ku),and TGH-Ⅴ(below 1 ku) using an ultrafiltration (UF) membrane bioreactor system based on the molecular weight cut-offs.The fraction of TGH-Ⅴ,exhibited the highest superoxide scavenging activity,was further purified using consecutive chromatographic methods including gel filtration,ion-exchange chromatography,and reverse-phase high performance liquid chromatography.The purified peptide had a strong scavenging ability on superoxide and the IC50 value was 4.6 μg·mL-1.And the molecular weight of the purified peptide was between 311.3-932.8 u determined by MS.关键词
尼罗罗非鱼/胶原蛋白/酶解/游离基清除肽Key words
Oreochromis niloticus/gelatin/enzymic hydrolysis/radical scavenging peptide分类
生物科学引用本文复制引用
曾名湧,郭瑶,刘尊英,董士远,赵元晖,崔文萱..尼罗罗非鱼鱼皮胶原蛋白中游离基清除肽的制备及其抗氧化活性[J].水产学报,2008,32(1):117-124,8.基金项目
Project of Qingdao City Science and Technology (03-1-HH-12) （03-1-HH-12）
青岛市科技攻关计划(03-1-HH-12) （03-1-HH-12）
