北京大学学报（医学版）2001，Vol.33Issue(2)：118-121,4.
周期性牵张力对人牙周膜细胞中成骨样细胞表型碱性磷酸酶和骨钙素mRNA表达的影响
Effect of cyclical mechanical force on expression of mRNAs for osteoblast-like marker proteins in human periodontal ligament cells in vitro
张丁 1李小彤 2傅民魁1
作者信息
- 1. 北京大学口腔医院正畸科，北京 100081
- 2. 北京大学口腔医院正畸科,
- 折叠
摘要
Abstract
Objective： To study the role of mechanical stress on osteoblast-like characteristics expressed by human periodontal ligament cells in vitro. Methods： Human periodontal ligament cells (hPDLC) were cultured by explant method. We investigated the gene expression for osteoblast-like marker proteins in periodontal ligament stimulated by cyclical mechanical force in vitro by in situ hybridization with non-radioisotopic oligonucleotide probe for alkline phosphatase (ALP) and osteocalcin (OCN). Results： Human periodontal ligament cells exhibited a stronger signal for ALP mRNA stimulated by cyclical mechanical force, and exhibited a moderately intense positive signal for OCN mRNA—OCN was a marker of differentiated osteoblast-like cells. Conclusion： These findings suggest that mechanical force plays role in differentiation from hPDLC to osteoblast-like cells.关键词
牙周组织/成骨细胞/碱性磷酸酶/代谢/骨钙素/代谢/间歇性牵张力/RNA,信使/代谢分类
医药卫生引用本文复制引用
张丁,李小彤,傅民魁..周期性牵张力对人牙周膜细胞中成骨样细胞表型碱性磷酸酶和骨钙素mRNA表达的影响[J].北京大学学报（医学版）,2001,33(2):118-121,4.
