哈尔滨工业大学学报（英文版）2008，Vol.15Issue(1)：118-123,6.
Development of real-time PCR method for rapid detection and quantification of Heterosigma akashiwo
Development of real-time PCR method for rapid detection and quantification of Heterosigma akashiwo
HE Shan-ying 1YU Zhi-gang 2MI Tie-zhu3
作者信息
- 1. College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China
- 2. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003 ,China
- 3. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266003, China
- 折叠
摘要
Abstract
To rapidly detect the harmful algae H. Akashiwo qualitatively and quantitatively, sequences of the 18S rDNA deduced from H. Akashiwo were used for designing species-specific primers, and a RFQ-PCR (Real-time Fluorescent Quantitative Polymerase Chain Reaction) method was developed for quantitative detection of H.akashiwo. Primer H. Akashiwo and TaqMan probe were designed, and the specificity of primer was checked with PCR. A calibration curve was constructed with cycle threshold value against visual counted cell number. And the value of the curve was tested with other H. Akashiwo samples, which were assayed with both the RFQ-PCR method and visual count under microscope.关键词
Heterosigma akashiwo/ fluorescent quantitative PCR/ molecular probe/ real-time detectionKey words
Heterosigma akashiwo/ fluorescent quantitative PCR/ molecular probe/ real-time detection分类
农业科技引用本文复制引用
HE Shan-ying,YU Zhi-gang,MI Tie-zhu..Development of real-time PCR method for rapid detection and quantification of Heterosigma akashiwo[J].哈尔滨工业大学学报（英文版）,2008,15(1):118-123,6.
