中华医学杂志（英文版）2002，Vol.115Issue(8)：1191-1195,5.
豚鼠内淋巴积水Ca2+-ATP酶的变化
Changes in Ca2+-ATPase in a guinea pig endolymphatic hydrops model
张素珍 1杨伟炎 1孙建和 1赵承军 1周娜1
作者信息
- 1. 中国人民解放军总医院耳鼻咽喉科,北京,100853
- 折叠
摘要
Abstract
Objective To investigate the localization of Ca2+-ATPase (Ca2+ pump) in the cochlear and its change after endolymphatic hydrops, and to study the relationship between compound action potential (CAP) threshold and the Ca2+-ATPase activety.Methods The left endolymphatic sac was ablated to induce endolymphatic hydrops in fourteen healthy guinea pigs with normal action potential thresholds measured after a sliver ball electrode placed on the round window. Ca2+-ATPase activity was studied cytochemically using a lead citrate reaction in control and hydropic ears. The reaction product was lead phosphate particles as an expression of Ca2+-ATPase activity, observed with an eletron microscope. Results Ca2+-ATPase activity is mainly found on the endolymphatic surface of Reissner's membrane, the stereocilia and cuticular plate of inner and outer hair cells, and along the infolded plasma membrane of strial marginal cells. CAP thresholds of filtered click are increased and Ca2+-ATPase activity significantly decreased after endolymphatic hydrops in the mentioned locations.Conclusions CAP thresholds are increased and Ca2+-ATPase activity are significantly decreased in the cochlea after endolymphatic hydrops. These results suggest that there is a negative correlation between them.关键词
Ca2+-ATP酶 毛细胞 组织化学Key words
Ca2+-ATPase/hair cell/histochemistry分类
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张素珍,杨伟炎,孙建和,赵承军,周娜..豚鼠内淋巴积水Ca2+-ATP酶的变化[J].中华医学杂志（英文版）,2002,115(8):1191-1195,5.
