中国免疫学杂志2001，Vol.17Issue(3)：119-122,4.
CTL对同种异体靶细胞杀伤作用的实验研究
The study of CTL's cytotoxicity towards allogeneous targets
摘要
Abstract
Objective:Investigate the relationship between C TL specificcytotoxicity and HLA allotype.Methods:The Epst ein-Barr-virus-transformed B lymphoblastoid cell lines(EBV-LCLs) which have different class-I HLA allotype were used as stimulators to stimulate the periph eral blood mononuclear cells (PBMC) proliferating into cytotoxic T lymphocytes(C TL),then the specific CTL was used to test cytotoxicity with different targets ( EBV-LCL mentioned above).Results:When the autologous EBV -LCL (donor 1) was used as stimulator,the CTL-1a obtained from the mixed lymph o cyte culture had cytotoxicity only targeting the EBV-LCL of donor 1,no cytotox icity targeting the EBV-LCL of donor 2.But when the allogeneous EBV-LCL (donor 2,has completely eifferent HLA-I type from donor 1)was used as stimulator,the C TL-1b showed efficient cytotoxicity to the EBV-LCL of donor 2,but very low cytotoxicity to the EBV-LCL of donor 1. Conclusion:①Immune cells with different MHC allotypes c an interact each other.②The TCRs recognize neither the antigen peptide,nor the MHC allotype determinant (MHC specific antigen determinant);what they recognize is the several information of the MHC-peptide complex surface comparised of th e conformation,the nature and disturbution of the surface charge of the MHC-pep t ide complex.③The so-called restrictive cytotoxicity of CTL results from MHC- peptide complex specifically activates the corresponding CD8+ T cell clone.关键词
CTL限制 性杀伤 HLA型别 TCR MHC-抗原肽复合物 表面信息分类
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房崇芸,吴雄文,韩军艳,刘敏,杨志章,梁智辉,龚非力..CTL对同种异体靶细胞杀伤作用的实验研究[J].中国免疫学杂志,2001,17(3):119-122,4.基金项目
本课题受国家科学基金重点课题（3983034）和武汉市科委“晨光计划”资助 （）
