生物医学工程研究2009，Vol.28Issue(2)：119-123,5.
尿素脂质体偶联抗血管内皮生长因子受体的制备及理化性质
The Preparation and Physicochemical Properties of Medical Urea Liposomes Coupled with Antibody of Vascular Endothelial Growth Factor Receptor
摘要
Abstract
To improve the therapeutic efficency of treatment hemangioma with reducing the side effects of medical urea by preparing medical immunoliposomes and to study its physicochemical properties.Medical urea immunoliposomes were prepared through monoclonal antibody of vascular endothelial growth factor receptor being coupled to medical urea liposomes by reverse phase evaporation method and glutarldehyde method .The entrapment efficiency and the association ratio of the medical urea immunoliposomes were determined. Morphologic characteristics of medical urea immunoliposomes were investigated by transmission electron microscope.The results of gross appearance showed that the medical urea immunoliposomes were ivory white suspension and they were consisted of spherical or similar to spherical large unilamellar vesicles,and the particle size was about 150～200 nm by transmission electron microscope.The structure of nucleolus-like was observed. The entrapment efficiency of the medical urea immunoliposomes was up to 54.4%.The association ratio of the medical urea immunoliposomes was up to 36.84%.It is certain that the preparation of medical urea immunoliposomes is practicable and successful and monoclonal antibody of vascular endothelial growth factor receptor can act as coupled antibody of immunoliposomes.The entrapment efficiency and the association ratio should be raised.关键词
医用尿素/免疫脂质体/血管内皮生长因子受体/理化性质/偶联率/包封率Key words
Medical urea/Immunoliposomes/Antibody of vascular endothelial growth factor receptor/Physicochemical properties/Association ratio/Entrapment efficiency分类
医药卫生引用本文复制引用
王志亮,段降龙,段明辉,杨安宁,徐心,李国威..尿素脂质体偶联抗血管内皮生长因子受体的制备及理化性质[J].生物医学工程研究,2009,28(2):119-123,5.基金项目
陕西省科技计划基金资助项目(2004K14G2(3)) （2004K14G2(3）
