中国临床康复2004，Vol.8Issue(6)：1196-1197,2.
泽泻醇提取物对高血糖小鼠血液生化指标及胰岛素的影响
Effects of rhizome alismatis extract on blood biochemical indices and insulin in hyperglycemic mice
杨新波 1黄正明 1曹文斌 1陈微 2陶建华 1陈红艳 1田文辉 1王建华1
作者信息
- 1. 北京军医学院药理教研室,北京市,100071
- 2. 山东胜利石油管理局滨海医院内科,山东省东营市,257000
- 折叠
摘要
Abstract
AIM:To explore the effects of rhizome alismatis extract(RAE) on blood glucose,blood lipid,pancreatic amylase and insulin in normal and diabetic mice so as to provide scientific basis for the cure of diabetes with rhizome alismatis and intervention of rehabilitation measures. METHODS:Twenty-nine male mice from Kunming were randomly divided into 4 groups:saline group, gliclazide group,RAE 10 g/kg group and RAE 20 g/kg group.Alloxan(90 mg/kg) was injected into the caudal veins of the mice to establish hyperglycemia models.Blood glucose,serum lipid and pancreatic amylase were determined using automatic biochemistry analysor, and serum insulin was determined with radioimmunoassay.The pathological changes of pancreas and islets were observed under light microscope with HE staining and paraffin-section method. RESULTS:RAE,20 g/kg ig,reduced the blood glucose in normal mice significantly[control group(7.6± 1.1) mmol/L;RAE 20 g/kg group (5.6± 1.5) mmol/L,P< 0.05,t=2.329].RAE,10 and 20 g/kg ig, also decreased blood glucose after 7-day repeated administration in alloxan-induced hyperglycemic mice[control group( 21.9± 2.8) mmol/L;RAE 10 g/kg group(15.7± 5.7) mmol/L,P< 0.05,t=2.817;RAE 20 g/kg group( 9.3± 3.9) mmol/L,( t=2.329,P< 0.05,t=3.118,P< 0.01) ].Pathological examination: both the number and the size of islet tissues were reduced in alloxan-induced diabetic mice,while there were no significant changes in those treated with RAE.RAE(20 g/kg) also increased the release of insulin in normal mice[control group(16.4± 4.0) μ U/L, RAE 20 g/kg group(24.6± 8.1) μ U/L]and alloxan mice[control group (13.5± 4.3) μ U/L,RAE 20 g/kg group(20.1± 9.1) μ U/L]. CONCLUSION:RAE possesses potent effects of lowering blood glucose and serum lipid,and protecting islets from injury.Its anti-hyperglycemic mechanism may be associated with promoting release of insulin from beta cells in islets. 关键词
泽泻/四氧嘧啶/血糖/胰岛素分类
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杨新波,黄正明,曹文斌,陈微,陶建华,陈红艳,田文辉,王建华..泽泻醇提取物对高血糖小鼠血液生化指标及胰岛素的影响[J].中国临床康复,2004,8(6):1196-1197,2.
