西北国防医学杂志2001，Vol.22Issue(2)：120-122,3.
pH值对低温肺保护液减轻体外循环未成熟肺损伤的影响
Effects of pH of hypothermic lung preservation solution on lung injury of immature dog during CPB
李庆新 1吕国祯 1易定华 2宿学家2
作者信息
- 1. 兰州军区兰州总医院心胸外科,
- 2. 第四军医大学西京医院心外科中心,
- 折叠
摘要
Abstract
Objective:To observe the effects of hypothermic lung preservation solution with different pH on the acute lung injury of immature dogs during cardiopulmonary bypass(CPB) and to investigate the optimal pH for hypothermic lung preservation solution.Methods:36 experimental infant dogs(30 d)were randomly divided into 6 groups.Group A was CPB alone.In group B to F,pulmonary artery was perfused with lung preservation solution at pH 6.6，7.0，7.4，7.8，8.2respectively.The pulmonary artery was clamped for 60 min and then reperfused for 60 min．The samples were collected,and MPO,MDA and NO were determined by myeloperoxidase method,thiobarbituric acid method and nitrate reductase method respectively.The water content of lung tissue was measured and the ultrastructure of lung tissue was observed.Results:MPO and MDA in group C,D,E F were much lower than those in gruup A (P＜0.05,P＜0.01,P＜0.01,P＜0.05 respectively).MPO and MDA in group E were the lowest.However,NO in group C to F were significantly higher than those in group A (P＜0.05,P＜0.01,P＜0.01,P＜0.05 respectively).The water contents of lung in group D and E were significantly lower than those in group A(P＜0.01,P＜0.01).At the same time,the injuries degree of type Ⅱ epithelial cells and endothelial cells in the lung blood vessel were decreased in the perfusion groups.Conclusion:The hypothermic preservation solution can decrease the acute lung injury of immature dog during CPB,and the optimal pH is7.8.关键词
体外循环/缺血再灌注损伤/肺
体外循环/缺血再灌注损伤/肺分类
医药卫生引用本文复制引用
李庆新,吕国祯,易定华,宿学家..pH值对低温肺保护液减轻体外循环未成熟肺损伤的影响[J].西北国防医学杂志,2001,22(2):120-122,3.
