作物学报2008，Vol.34Issue(7)：1206-1212,7.
苏丹草高梁及其杂种的细胞遗传学研究
Cytogenetical Studies on Sorghum (S. bicolor), Sudangrass (S. sudanense), and Their Hybrid
摘要
Abstract
Sudangrass (Sorghum sudanense) and sorghum (S. bicolor) are two members of the genus Sorghum (Gramineae).Their hybrids have exhibited favorable forage yields and overall improved quality and disease resistance. They are widely used in aquaculture, production of livestock food and environmental protection, but some disagreements exist as to whether they actually belong to the same species. The Karyotypes of two sudangrass varieties, two sorghum varieties, three sorghum-sudangrass F1 hy-brids were analyzed by the method of cell wall degradation and hypotonic treatment for chromosome preparations, and the meio-sis behavior of three F1 hybrids and the chromosome numbers of two F2 hybrids were observed. The result showed that the karyo-typic types of sorghum and sudangrass were 1A, and the karyotype formula was 2n=18m+2sm (sat) for Sa, 2n=20m for 3042A and 3042A×Sa F1, and 2n=20m(sat) for the others. The difference of chromosomes among three groups (i.e. sudangrass, sorghum,and sorghum-sudangrass hybrid F1) were not significant in terms of absolute long arm, absolute short arm, absolute total length,arm ratio (l/s), relative total length (P>0.05); the change of chromosome length was not obvious in sorghum and sudangrass. The paired chromosome configuration of sorghum-sudangrass hybrid F1 in pollen mother cells at diakinesis and metaphaselwas 2n=2x=20(10Ⅱ) and the chromosome pairing behavior was regular, but the frequencies of rod bivalent of Tx623A×S722 F1,3042A×Sa F1, and Tx623A×Sa F1 were 4.887, 5.710, and 5.126, respectively; those of ring bivalent were 5.113, 4.290, and 4.874, respectively. At anaphase Ⅰ, the paired chromosomes of sorghum-sudangrass hybrid F1 could separate from each other. The chromosome numbers of F<,2> hybrid were 20 (2n=20). Therefore, the sudangrass/sorghum relationship is sufficiently close.关键词
苏丹草/高粱/杂交种/染色体/细胞遗传学Key words
Sudangrass [Sorghum sudanense (Piper) Stapf]/Sorghum [Sorghum bicolor (L.) Moench]/Karyotypes/Chromosome/Cytogenetics分类
农业科技引用本文复制引用
詹秋文,朱立猛,吴娟娟,张伟,张天真..苏丹草高梁及其杂种的细胞遗传学研究[J].作物学报,2008,34(7):1206-1212,7.基金项目
Supported by Anhui Provincial Natural Science Foundation (01041102), A key project of scientific and technical attack plan of Anhui Province (06013104B), A Key Project Grant from Anhui Science & Technology Department (04023069), Outstanding talent subsidy of 10th five-year plan of higher education of Anhui Province(200579),and Key Subject Foundation of Anhui Science and Technology University (YZD2004-03). （01041102）
