医疗卫生装备2009，Vol.30Issue(7)：12-13,16,3.
肝组织工程支架评价方法研究
Study on Evaluation Method of Scaffold for the Use of Liver Tissue Engineering
摘要
Abstract
Obiective To set up a method of scaffold evaluation using human cell line as seed cells and screen appropriate scaffold for live tissue engineering, Methods HepG2 cells were plated onto biodegradable polymer scaffolds: PLGA, 3% chitosan (3%CS) and 2% silk fibroin (2%SF), and cultured in vitro. The growth, distribution and function of HepG2 cells in the scaffolds were evaluated using MTT assay, H.E. staining, and urea assay kit. Results HepG2 cells plated on the three scaffolds maintained a proliferative state. In contrast, the cells on the 2%SF proliferated strongly, while the cells on the PLGA and chitin proliferated poorly. Histological examination showed that HepG2 cells distributed evenly on the 2%SF scaffold with a high amount, while few cells could be found on the PLGA and ehitin at day 7. Cell function assay showed that HepG2 cells on the 2%SF and PLGA exhibited slower decrease of urea synthesis compared to HepG2 cells on the chitosan. Conclusion The three scaffolds have good biocompatibility. In contrast, 2%SF scaffold is more appropriate for liver tissue engineering. This method may be used for scale screening of scaffolds for liver tissue engineering.关键词
PLGA/壳聚糖/丝素/HepG2细胞/生物材料评价Key words
PLGA/3%CS/2%SF/HepG2/evaluation of biomaterials分类
医药卫生引用本文复制引用
刘巨超,赵云山,刘广民,张博峰,余振定,徐迎新..肝组织工程支架评价方法研究[J].医疗卫生装备,2009,30(7):12-13,16,3.基金项目
国家自然科学基金资助项目(50573091) （50573091）
