临床医学工程2009，Vol.16Issue(7)：12-14,3.
PPAR-γ配体对巨噬细胞源泡沫细胞形成及TLR-2、TLR-4表达的影响
Effects of PPAR-γ, Ligand on the Mice Peritoneal Macrophage Foam Cells Formation and TLR-2, TLR-4 Expression Induced by OxLDL
李飞 1袁勇 1张励庭 1冯力 1黄炫生 1李明星1
作者信息
- 1. 中山大学附属中山医院心血管科,广东,中山,528400
- 折叠
摘要
Abstract
Objective To observe the effects of PPAR-y ligand, GW1929 on the mice peritoneal macrophage foam cells formation and TLR-2, TLR-4 expression induced by oxLDL. Methods The mice peritoneal macrophages were pretreated with oxLDL (50 μg/Ml) and GW1929 (20μmol/L) respectively for 24 h. Then they were stained by oil red O to observe foam cells formation. Then confocal fluorescence microscopy was used to observe effects of GW1929 on foam cells TLR-2 and TLR-4 expressions. Results The mice peritoneal macrophages of control group and GW1929 group are not almost stained by oil red O, but the macrophages of oxLDL group become dark red after stained by oil red O, and the cells color of oxLDL+GWI929 group is thinner than that of oxLDL group. Expressions of TLR-2, TLR-4 in oxLDL group are higher than those in control and GW1929 group obviously, but expressions in oxLDL+GW1929 group are lower than oxLDL group respective-ly. Conclusion PPAR-γ ligand, GW1929 probably stable atheromatons plaque through inhibiting foam cells foamation and expressions of TLR-2, TLR-4.关键词
动脉粥样硬化/炎症反应/过氧化物酶体增生物激活受体γ/Toll样受体/巨噬细胞Key words
Atherosclerosis/Inflammation/Peroxisome proliferator activated receptors T/Toll-Like Receptors/Maerophages分类
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李飞,袁勇,张励庭,冯力,黄炫生,李明星..PPAR-γ配体对巨噬细胞源泡沫细胞形成及TLR-2、TLR-4表达的影响[J].临床医学工程,2009,16(7):12-14,3.
