中国兽医杂志2000，Vol.26Issue(11)：12-14,3.
不同型魏氏梭菌菌体蛋白和膜蛋白SDS-PAGE电泳图谱分析
SDS-PAGE analysis of cell proteins and membrane proteins of Clostridium perfringens
杨明凡 1田永军 2崔保安 1王学斌 1苗舜尧1
作者信息
- 1. 河南农业大学牧医工程学院，河南 郑州 450002
- 2. 河南省畜牧局，河南 郑州 450003
- 折叠
摘要
Abstract
The cell proteins of different types of Cl.perfringens produce reproducible electrophoretic patterns. In the electrophoretic patterns of cell proteins Cl. perfringens type A didn't have the 54.9 KD band; type C didn't have the 107.8 KD and 36.2 KD bands and type D didn't have the 107.8 KD and 66.6 KD bands. So the electrophoretic patterns of cell proteins in polyacrylamide gel were used for the rapid identification and classification of Cl. perfringens. And the electrophoretic patterns of membrane proteins of the different Cl. perfringens were specific. In the electrophoretic patterns of membrane proteins only type B had the 94.7 KD band but didn't have the 51 KD band; type C didn't have the 74.2 KD band; only type D had the 24.3 KD band but didn't have the 31 KD band and the 38.1 KD band was faint. This method may also be used for the identification of Cl. perfringens.关键词
魏氏梭菌/菌体蛋白/膜蛋白/SDS-聚丙烯酰胺电泳(SDS-PAGE)技术Key words
Clostridium perfringens/cell proteins/membrane proteins/Sodium Dodecyl SulphatePolyacrylamide Gel Electrophoresis分类
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杨明凡,田永军,崔保安,王学斌,苗舜尧..不同型魏氏梭菌菌体蛋白和膜蛋白SDS-PAGE电泳图谱分析[J].中国兽医杂志,2000,26(11):12-14,3.
