首页|期刊导航|中华医学杂志（英文版）|Correlation of expression of preprothyrotropin-releasing hormone and receptor with rat testis development
中华医学杂志（英文版）2002，Vol.115Issue(1)：12-16,5.
Correlation of expression of preprothyrotropin-releasing hormone and receptor with rat testis development
Correlation of expression of preprothyrotropin-releasing hormone and receptor with rat testis development
摘要
Abstract
Objective To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and TRH receptor (TRH-R), and their role in the development of rat testis.Methods Oligonucleotide primers were designed from the sequences of rat hypothalamus prepro TRH (ppTRH) and pituitary TRH-R cDNA for reverse transcription polymerase chain reaction (RT-PCR). Specific fragments of ppTRH and TRH-R cDNA were cloned and sequenced. Expression plasmids containing ppTRH and TRH-R genes were then constructed, and expression was found in E.coli DH5-α. ppTRH and TRH-R mRNA in the testis was quantitated in RNA samples prepared from rats at different developmental stages by real time quantitative RT-PCR.Results The quantitative analyses demonstrated that no ppTRH and TRH mRNA could be detected at the earliest stage (day 8). ppTRH and TRH mRNA signals were detected on day 15 and increased progressively on days 20, 35, 60 and 90. Conclusion Our results suggest that rat testis could specifically express TRH and TRH-R, and the transcriptions of ppTRH and TRH-R genes in the rat testis were development-dependent. The acquirement of expressed products for ppTRH and TRH-R can be used for further research on the physiological significance of TRH and TRH-R expression in rat testis.关键词
prepro thyrotrophin-releasing hormone/thyrotrophin-releasing hormone receptor/rat testis/real time quantitative RT-PCRKey words
prepro thyrotrophin-releasing hormone/thyrotrophin-releasing hormone receptor/rat testis/real time quantitative RT-PCR分类
医药卫生引用本文复制引用
李臻,张远强,刘新平,许若军..Correlation of expression of preprothyrotropin-releasing hormone and receptor with rat testis development[J].中华医学杂志（英文版）,2002,115(1):12-16,5.基金项目
This work was supported by the National Natural Science Foundation of China (No. 39870109) and the Military Medical Research Foundation (No. 98M106). （No. 39870109）
