中国医学工程2002，Vol.10Issue(6)：12-16,5.
乳糖化修饰纳米基因载体的肝靶向性研究
STUDIES OF TARGETING EFFECTS OF LACTOSAMINATED NANOPARTICLE GENE CARRIER ON LIVER
张阳德 1王光锁 2潘一峰 1龚连生 2刘金波1
作者信息
- 1. 中南大学卫生部肝胆肠外科研究中心,长沙,410008
- 2. 中国卫生部纳米生物技术重点实验室
- 折叠
摘要
Abstract
Objective:To compare the targeting effects of lactosarninated alginate(AlgNP)、polyethylene glycol - coated hydroxyapatite- poly- L- lysine nanoparticles (PLL- PCHNP)and relative nonlactosaminated ones load ed with exogenous gene on liver via peripheral intravenous route. Methods:Preparation of AlgNP based on control of gelification phenomenon of algiante by calcium ions and HA- PLLNP with collosol - gel method, both further modified with lactosaminated - poly- L - lysine synthesized by reductive lactosamination . We used pEGFPCl as the reporter gene to establish receptor- mediated and positive liver targeting nanoparticles- gene model. The potential of adsorbing DNA on nanoparticles was analysed by electrophoresis and spectrophotometer. Then different complexes were transferred into the rat's body by peripheral intravenous route and their targeting characteristics in liver were investigated by using radioisotope tracing assay. Results: PCHNP presented as needle - like particles with a diameter of 20nm by TEM and could be effectively combined with PLL. The diameter of AlgNP was 280nm. Agarpse gel electrophoresis showed both nanoparticles could effectively combine with DNA and the optimal proportion of PLLPCHNP and DNA was 30:1 (w/w); DNA mixed ratio of AlgPLL was 68.3 % by spectrophotometer. The radioactivities in liver for the two lactosaminated nanoparticles were higher than the nonlactosaminated ones. No statistic difference between AlgNP and AlgLacNP could be found . Conclusions: Lactosaminated naroparticles can target to liver more effectively by peripheral intravenous route than nonlactosaminated ones, which is closely concerned with the characteritics of the nanopartide complex.关键词
Lactosaminated Nanoparticle/Alginate Nanoparticle/Hydroxyapatite- PLL Nanoparticle/pEGFPC1 Plasmid/Active Liver Targeting/Radioisotope Tracing AssayKey words
Lactosaminated Nanoparticle/Alginate Nanoparticle/Hydroxyapatite- PLL Nanoparticle/pEGFPC1 Plasmid/Active Liver Targeting/Radioisotope Tracing Assay引用本文复制引用
张阳德,王光锁,潘一峰,龚连生,刘金波..乳糖化修饰纳米基因载体的肝靶向性研究[J].中国医学工程,2002,10(6):12-16,5.
