中国现代医学杂志2004，Vol.14Issue(1)：12-16,20,6.
采用反义寡核苷酸探讨HSP70对活性氧所致心肌细胞损伤的影响
Study of effect of HSP70 on acute injury of cardiomyocyte induced by hydrogen peroxide using antisense oligodeoxynucleotides
摘要
Abstract
Objective:To investigate the protective effect of HSP70 against acute injury of cardiomyocyte induced by H2O2. Methods: Neonatal rat cardiomyocytes were treated with heat shock (42 C 30 rmin, recovery for 12hfs) to induce expression of HSP70 and HSP70 antisense oligodeoxynucleotides were transfected to block the expression of HSP70. LDH release rate and total protein synthesis were applied to evaluate the injury of neonatal rat cardiomyocyte induced by H2O2 (0.5 mmol/L). Results: H2O2 could significantly increased LDH release and inhibit totalprotein synthesis. By inducing the expression of HSPs,heat shock pretreatment significantly reduced LDH release and attenuated the inhibition of total protein synthesis induced by H2O2. Moreover, HSP70 antisense oligodeoxynucleotides remarkably blocked the protective effect of heat shock pretreatment against the cellular injuries induced by H2 O2. Conclusion: HSP70 plays a most important role in cytoprotection of heat shock pretreatment against acute injury of cardiomyocyte mediated by H2O2.关键词
热休克蛋白70/反义寡核苷酸/心肌细胞/活性氧Key words
HSP70/antisense oligodeoxynucleotides/cardiomyocyte/hydrogen peroxide分类
医药卫生引用本文复制引用
王慷慨,邓恭华,涂自智,易宇欣,王浩,刘可,刘梅冬,肖献忠..采用反义寡核苷酸探讨HSP70对活性氧所致心肌细胞损伤的影响[J].中国现代医学杂志,2004,14(1):12-16,20,6.基金项目
This work was supported by grants from the Special Funds for Major State Basic Research of China ( No. G2000056908) ,the National Natural Science Foundation of China (No. 30000069 （ No. G2000056908）
30300177) and the Special Funds of the Ministry of Education for Ph D (No. （）
