南华大学学报（自然科学版）2004，Vol.18Issue(3)：12-17,6.
吡啰红Y共振光散射法测定纳克级核酸
Determination of Nucleic Acids at Nanogram Levels with Pyronine Y by a Resonance Light-Scattering Technique
摘要
Abstract
Based on the enhancement of resonance light scattering(RLS) of Pyronine Y(PY) by nucleic acids,a new quantitative determination method for nucleic acids in the nanogram range has been developed.In the pH range 11.5-12.0,the RLS of PY is greatly enhanced by nucleic acids,with the maximum scattering peak located at 364.0nm.The enhanced intensity of RLS,△IRLS,was found to be proportional to the concentration of nucleic acids in the range 27.0～625 ng/mL for fish sperm DNA,39.0～500 ng/mL for calf thymus DNA and 59.0～375 ng/mL for yeast RNA.The limits of determination were 8.1 ng/mL for fish sperm DNA,11.7 ng/mL for calf thymus DNA and 17.7 ng/mL for yeast RNA.Six synthetic samples were determined satisfactorily.The assay is convenient,rapid and inexpensive.关键词
吡啰红Y/核酸/共振光散射法Key words
pyronine Y/nucleic acids/resonance light scattering(RLS)分类
化学化工引用本文复制引用
王永生,肖锡林,李贵荣,吕昌银..吡啰红Y共振光散射法测定纳克级核酸[J].南华大学学报（自然科学版）,2004,18(3):12-17,6.基金项目
the Sciences Research Program of Educational Department of Hunan Province (01C213) （01C213）
