首页|期刊导航|南京医科大学学报(英文版)|Whole-cell recordings of voltage-gated Calcium, Potassium and Sodium currents in acutely isolated hippocampal pyramidal neurons
南京医科大学学报(英文版)2009，Vol.23Issue(2)：122-126,5.
Whole-cell recordings of voltage-gated Calcium, Potassium and Sodium currents in acutely isolated hippocampal pyramidal neurons
Whole-cell recordings of voltage-gated Calcium, Potassium and Sodium currents in acutely isolated hippocampal pyramidal neurons
摘要
Abstract
Objective:To record Calcium, Potassium and Sodium currents in acutely isolated hippocampal pyramidal neurons. Methods:Hip-pocampal CA3 neurons were freshly isolated by 1 mg protease/3 ml SES and mechanical trituration with polished pipettes of progressively smaller tip diameters. Patch clamp technique in whole-cell mode was employed to record voltage-gated channel currents. Results:The procedure dissociated hippocampal neurons, preserving apical dendrites and several basal dendrites, without impairing the electrical characteristics of the neurons. Whole-cell patch clamp configuration was successfully used to record voltage-gated Ca2+ currents, delayed rectifier K+ current and voltage-gated Na+ currents. Conclusion:Protease combined with mechanical trituration may be used for the dissociation of neurons from rat hippocampus. Voltage-gated channels currents could be recorded using a patch clamp technique.关键词
patch clamp/hippocampus/voltage-gated channels/whole-cellKey words
patch clamp/hippocampus/voltage-gated channels/whole-cell分类
医药卫生引用本文复制引用
Shuyun Huang,Qing Cai,Weitian Liu,Xiaoling Wang,Tao Wang..Whole-cell recordings of voltage-gated Calcium, Potassium and Sodium currents in acutely isolated hippocampal pyramidal neurons[J].南京医科大学学报(英文版),2009,23(2):122-126,5.基金项目
This work was supported by Science Development Foundation of Tianjin Institute of Education(20070301). （20070301）
