中国医学科学院学报2001，Vol.23Issue(2)：123-126,4.
重组CC家族趋化因子RANTES的原核表达及趋化活性分析
Expression in E.coli and Chemotactic Activity Analyzing of Recombinant CC-chemokine RANTES
摘要
Abstract
Objective To further understand the structure and function of RANTES and provide basis for research
for application. Methods PCR product corresponding to region encoding mature RANTES protein was
cloned into E.coli expression vector;, purified recombinant protein was obtained by heparin affinity chro
matography and its chemotactic activity was determined by Boyden chamber. Results The recombinant
RANTES was expressed in E.coli with high efficiency. Purified protein showed chemotactic activity to pe
ripheral blood lymphocytes, U937 cells, and CCR4 stable-transfected HEK293 cells. Conclusion The puri
fled recombinant protein exerted chemotactic activity on PBL and cultured cell lines, and we have estab
lished a experimental system for further study of structure and function of RANTES.关键词
趋化因子RANTES原核表达趋化活性分类
生物科学引用本文复制引用
孙荣华,王应,钟英诚,马大龙..重组CC家族趋化因子RANTES的原核表达及趋化活性分析[J].中国医学科学院学报,2001,23(2):123-126,4.
