第一军医大学学报2003，Vol.23Issue(12)：1242-1244,3.
BALB/c小鼠腹腔接种HBV表面S抗原疫苗引起的CTL反应
Cytoxic T lymphocyte responses to hepatitis B virus small surface antigen in BALB/c mice
施理 1刘树人 1易学瑞 1孔祥平 1张宜俊1
作者信息
- 1. 458医院全军肝病中心,广东,广州,510602
- 折叠
摘要
Abstract
Objective To observe cytotoxic T lymphocytes (CTL) responses induced by immunization of mice with hepatis B small surface antigen (S-HBsAg). Methods BALB/c(H-2d) mice were injected intraperitoneally with S-HBsAg at the doses ranging from 0-5 μg with a booster injection 2 weeks later. Four weeks after the booster immunization, the specific cytotoxic reactivity of the spleen lymphocytes isolated from the immunized mice, following stimulation by S-HBsAg specific CTL epitope peptide in vitro, with 51Cr-labeled P815 cells treated with the peptide was observed. Results The percentage of specific release of 51Cr in mice immunized with S-HBsAg at the doses of 0, 0.65, 1.25, 2.5, and 5 μg was 31.21%±9.23%, 42.36%±19.32%, 91.21%±22.97%, 69.25%±24.13% and 51.49%±21.661% respectively. In mice receiving the only primary vaccination, the corresponding percentage was 27.34%±14.25%, 32.27%±15.35%, 56.28%±24.35%, 44.34%±18.65% and 40.76±56% respectively. Conclusion Immunization with S-HBsAg efficiently elicits CTL response in BALB/c mice in a dose-dependent manner and enhanced by booster immunization.关键词
HBV表面S抗原疫苗/BALB/c小鼠/免疫接种/细胞毒T淋巴细胞Key words
hepatitis B virus/small surface antigen/BALB/c mice/immunization/cytotoxic T lymphocytes分类
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施理,刘树人,易学瑞,孔祥平,张宜俊..BALB/c小鼠腹腔接种HBV表面S抗原疫苗引起的CTL反应[J].第一军医大学学报,2003,23(12):1242-1244,3.
