中国农业科学Issue(1)：125-132,8.
Oocyte Pickup from Live Cows Through Laparoscopic Guided Aspiration
Oocyte Pickup from Live Cows Through Laparoscopic Guided Aspiration
Ni Hemin 1Guo Yong 1Zhu Yuding 2Liu Yunhai 2Dou Zhongying3
作者信息
- 1. Beijing Agricultural College
- 2. Institute of Animal Science, CAAS,Beijing l00094
- 3. Northwestem Agricultural University
- 折叠
摘要
Abstract
In this experiment, the bovine follicular oocytes were aspirated from the ovaries of Chinese Holsteins with laparoscope made in China. The results were as following: for identifying the suitable negative aspiration pressure, six different pressures (50, 100, 150, 200, 250 and 300mmHg)were tested. The aspiration pressure of 100mmHg was the best. Its oocyte recovery rate was 37. 2%, and G I , G Ⅱ oocyte rate was 89. 5%. The heifers were picked up by laparoscope once or twice a week. Each heifer was collected with 2. 4 oocytes once a week or 4. 4 oocytes twice a week.Its oocyte recovery rate was 48. 0% and the G Ⅰ ,G Ⅱ oocyte rate was 93. 5%. In addition, 1.9 oocytes were collected from each cow once a week or 5.4 oocytes from each cow twice a week. Its oocyte recovery rate was 51.7% and the G Ⅰ , G Ⅱ oocyte rate was 85. 1%. It showed that it was possible to pick up bovine oocyte twice a week. Two cows were picked up twice a week for several weeks(53 times). 268 follicles were aspirated(5.1 follicles per cow per time), and 141 oocytes were recovered(2.7 oocytes per cow per time). The oocyte recovery rate was 52.5%, and the G Ⅰ , G Ⅱ oocyte rate was 85. 1%. It was advisable to pick up oocytes twice a week continuously. Some cows in estrous cycles were superovulated with PMSG(500IU). Each of them could be recovered 2.3 follicles and 1.1 oocytes, the others were superovulated with FSH(0. 7mg) , each of them could be aspirated with 4.4 follicles and 2.3 oocytes. It was obvious that the effect of OPU(oocyte pick up) by superovulation with FSH was much better than that with PMSG. The best time for OPU with laparoscope was at the beginning of cow's estrous cycles. At the first day of their estrus, each of them could be averagely aspirated with 8 follicles and 5.7 oocytes.关键词
Bovine/Oocyte pick up/LaparoscopeKey words
Bovine/Oocyte pick up/Laparoscope引用本文复制引用
Ni Hemin,Guo Yong,Zhu Yuding,Liu Yunhai,Dou Zhongying..Oocyte Pickup from Live Cows Through Laparoscopic Guided Aspiration[J].中国农业科学,2000,(1):125-132,8.
