中国病理生理杂志2000，Vol.16Issue(12)：1260-1262,3.
金属硫蛋白参与碱性成纤维细胞生长因子的心肌细胞保护作用
Metallothionein involvement in myocardial protection of basic fibroblast growth factor
摘要
Abstract
AIM: To observe whether metallothionein plays a role in cardiac protective effect of basic fibroblast growth factor on anoxic/reperfusion (A/R) injury in cultured cardiomyocytes and study the possible mechanism of cardiac protection by bFGF.METHODS: The present study made the model of myocyte A/R injury after having a 24 h incubation by bFGF( 10-10、10-9、10-s mol/L) and bFGF( 10-9 mol/L) + PD098059 respectively. We measured the levels of MT and MDA in myocytes, and the changes of LDH and protein in cultured medium. We also counted the number of viable cell in groups. RESULTS: The contents of myocardial MT were significantly increased after treatment by bFGF. The levels of MT in I0-l0 mol/L、10-9 mol/L and 10-8 mol/L bFGF treated groups increased 54 %、 62%、 76% respectively, compared with the A/R group, and the number of viable cell were also greatly increased, LDH and protein leakage in cultured medium and MDA contents in myocyte were dramatically decreased in bFGF treated groups. All the protection were completely disappeared with the inhibition of MT production with PD098059, theinhibitor of mitogen- activated protein kinase(MAPK). CONCLUSION: MT involves in the protection of bFGF in cultured cardiomyocytes. It might be related with activation of MAPKase.关键词
金属硫蛋白/成纤维细胞生长因子,碱性/低氧/再灌注Key words
Metallothionein/Fibroblast growth factor, basic/Anoxia/Reperfusion分类
医药卫生引用本文复制引用
李淑莲,王晓红,符民桂,庞永政,唐朝枢..金属硫蛋白参与碱性成纤维细胞生长因子的心肌细胞保护作用[J].中国病理生理杂志,2000,16(12):1260-1262,3.基金项目
国家自然科学基金资助课题(No.39730220) （No.39730220）
