首页|期刊导航|中国农业科学（英文版）|Development and Application of a PCR Approach for Detection of Bovis, Sheep, Pig, and Chicken Derived Materials in Feedstuff
中国农业科学（英文版）2008，Vol.7Issue(10)：1260-1266,7.
Development and Application of a PCR Approach for Detection of Bovis, Sheep, Pig, and Chicken Derived Materials in Feedstuff
Development and Application of a PCR Approach for Detection of Bovis, Sheep, Pig, and Chicken Derived Materials in Feedstuff
摘要
Abstract
A PCR method for detection of bovis, sheep, pig, and chicken derived materials in feedstuff was established, and the existing method was improved according to the research on general primer and species-specific primers. First, general primer designed according to 16S rRNA gene sequence of bovis, sheep, pig, chicken, fish, and horse mtDNA was used for primary detection of animal derived materials in feedstuff. Species-specific primers designed according to conserved sequence of mtDNA of bovis, sheep, pig, and chicken were used for amplification of a 271, 274, 149, and 266 bp fragment, respectively. Further confirmation of the detection result was then carried out. PCR method for detection of animal derived materials in unknown feedstuff was developed by using general primer, relevant PCR system, and PCR condition. Also a PCR method for detection of each species (bovines, sheep, pig, and chicken) was designed by using our species-specific primers. High sensitivity and specificity of our method were confirmed with a minimum detection level of 0.1%. Method for detection of animal derived materials in this research is not only cheap and easy for operation but also precise and reliable results can be obtained. It could be one of the effective methods for the detection of animal derived materials in feedstuff.关键词
animal feedstuff/sensitivity and specificity/PCR/primerKey words
animal feedstuff/sensitivity and specificity/PCR/primer分类
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LUO Jia-qin,WANG Jia-qi,BU Deng-pan,LI Dan,WANG Li,WEI Hong-yang,ZHOU Ling-yun..Development and Application of a PCR Approach for Detection of Bovis, Sheep, Pig, and Chicken Derived Materials in Feedstuff[J].中国农业科学（英文版）,2008,7(10):1260-1266,7.基金项目
This paper is translated from its Chinese version in Scientia Agricultura Sinica. The study was supported by grants from Ministry of Sciences and Technology, China (2006BAD12B08,2006BAD04A03) and Ministry of Agriculture, China (Nyhzx07-036). （2006BAD12B08,2006BAD04A03）
