中德临床肿瘤学杂志(英文版)2003，Vol.2Issue(3)：126-131,6.
信号调节蛋白α1在肝细胞癌中的作用
The Possible Role of Signal Regulatory Protein α1 in Hepatocellular Carcinoma
单云峰 1康斌 1李宝安 1鄢和新 1周伟平 1吴孟超 1王红阳1
作者信息
- 1. 东方肝胆外科医院国际合作信号转导研究室,200438
- 折叠
摘要
Abstract
Objective: To study the relationship between signal regulatory protein α1 (SIRPα1) and hep-atocellular carcinoma (HCC) and explore the possible molecular mechanisms. Methods: Total RNA wasextracted from normal hepatocyte cell line Hs680, Hepatocellular carcinoma cell lines HepG2, H4-H7,Chang liver and SK-Hep1. The SIRPα1 expression was determine d by Northern blot. The mRNA andprotein expression of SIRPα1 was detected in 42 pathologically confirmed hepatocellular carcinoma tissuesamples and 6 normal liver tissues by Northern blot and immunohistochemical staining. The association ofSIRPα1 and HGF was determined in HuH7. Results: SIRPα1 was highly expressed in Hs680, HepG2 andH4-H7, but weakly expressed in SK-Hep1 and Chang liver cell lines. Northern blot and Western blot bothdetected the reduced expression of SIRPα1 in hepatocellular carcinoma tissue as compare to paracancerousand normal tissues. In vitro experiments showed HGF stimulation elevated SIRPα1 phosphorylation. Over-expression of wild type SIRPα1 promoted cell proliferation in the presence of HGF. Conclusion: SIRPα1might be involved in HCC development and metastasis. The underlying mechanisms may be related toregulatory tyrosine phosphorylation of SIRPα1 by HGF stimulation.关键词
信号调节蛋白/抑制性受体/肝细胞癌/肝细胞生长因子Key words
signal regulatory protein/Inhibitory receptor/Hepatocellular carcinoma/HGF分类
医药卫生引用本文复制引用
单云峰,康斌,李宝安,鄢和新,周伟平,吴孟超,王红阳..信号调节蛋白α1在肝细胞癌中的作用[J].中德临床肿瘤学杂志(英文版),2003,2(3):126-131,6.
