中国病理生理杂志2000，Vol.16Issue(12)：1263-1266,4.
大鼠短暂全脑缺血再灌后谷氨酸透过血脑屏障的变化
Glutamate transport across blood brain barrier after transient global ischemia/reperfusion in rats
摘要
Abstract
AIM: To study the change of glutamate (Glu) transport across blood brain barrier ( BBB ) in rat following forebrain ischemia/reperfusion. METHODS: BBB unidirectional transfer constant ( Ki ) for [3H] - Glu in rat hippocampus, cerebral cortex and striatum were determined after rats were subjected to cerebral ischemia 10 min ( two - carotid occlusion plus hypovolemic hypotension) followed by 0.17, 2, 6 and 24 h of reperfusion. The recovery of [3H] - Glu in cerebrum was also determined after intracerebral injection of [3H] - Glu in another experiment. RESULTS: Compared with control rat brain, Ki for [3H] -Glu significantly( P < 0.05) decreased at 10 ain cerebral ischemia followed by 0.17, 2 and 6 h of reperfusion. At 5 min after intracerebrally injecting [3H] - Glu , recovery of [3H] - Glu in control rat brain was 23.83%. The result indicted that there is a Glu efflux mechanism on BBB. This efflux was not significantly inhibited by pretreatment of 200 mg/L probenecid. After 10 ain cerebral ischemia followed by 2 h of reperfusion, the recovery( 13.13 % ) was significantly lower than contro( P < 0.05), its recovery was only 55 % of the control. The result indicated that cerebral ischemia/reperfusion may enhanced the effiux of [3H] -Glu from brain. CONCLUSION: Cerebral ischemia/reperfusion significantly reduced Glu BBB transport from plasma to brain and enhanced effiux of Glu from brain.关键词
谷氨酸盐类/血脑屏障/脑缺血,暂时性/再灌注Key words
Glutamates/Bl ood- brain barrier/Cerebral ischemia, transient/Reperfusion分类
医药卫生引用本文复制引用
刘晓东,刘国卿..大鼠短暂全脑缺血再灌后谷氨酸透过血脑屏障的变化[J].中国病理生理杂志,2000,16(12):1263-1266,4.基金项目
国家自然科学基金资助项目(No．39870885) （No．39870885）
