首页|期刊导航|中国农业科学（英文版）|The Helper Activities of Different Avian Viruses for Propagation of Recombinant Avian Adeno-Associated Virus
中国农业科学（英文版）2007，Vol.6Issue(10)：1269-1274,6.
The Helper Activities of Different Avian Viruses for Propagation of Recombinant Avian Adeno-Associated Virus
The Helper Activities of Different Avian Viruses for Propagation of Recombinant Avian Adeno-Associated Virus
摘要
Abstract
To compare the helper activities of different avian viruses for propagation of recombinant avian adeno-associated virus (rAAAV), AAV-293 cells were cotransfected with the AAAV vector pAITR-GFP containing green fluorescent protein (GFP) gene, the AAAV helper vector pcDNA-ARC expressing the rep and cap genes, and the adenovirus helper vector pHelper expressing Ad5 E2A, E4, and VA-RNA genes. Chicken embryonic fibroblast (CEF) or chicken embryonic liver (CEL) cells were cotransfected with the AAAV vector and the AAAV helper vector, followed by infection with Marek's disease virus (MDV), avian adenovirus, chicken embryo lethal orphan (CELO) virus or infectious bursal disease virus (IBDV). Infectious rAAAV particles generated by the two strategies were harvested and titrated on CEF and CEL cells. A significantly higher viral titer was obtained with the helper activity provided by the pHelper vector than by MDV or CELO virus. Further experiments showed that rAAAV-mediated green fluorescent protein (gfp) expression was overtly enhanced by MDV or CELO virus super infection or treatment with sodium butyric acid, but not by IBDV super infection. These data demonstrated that MDV and CELO viruses could provide weak helper activity for propagation of rAAAV, and rAAAV-mediated transgene expression could be enhanced by super infection with the helper viruses.关键词
recombinant avian adeno-associated virus (rAAAV)/ helper virusesKey words
recombinant avian adeno-associated virus (rAAAV)/ helper viruses分类
农业科技引用本文复制引用
WANG An-ping,SUN Huai-chang,WANG Jian-ye,WANG Yong-juan,YUAN Wei-feng..The Helper Activities of Different Avian Viruses for Propagation of Recombinant Avian Adeno-Associated Virus[J].中国农业科学（英文版）,2007,6(10):1269-1274,6.基金项目
This work was supported by the National Natural Science Foundation of China (30571373). （30571373）
