中国药科大学学报2004，Vol.35Issue(2)：127-130,4.
HPLC测定四季青中5种酚酸类成分的含量
Simultaneous Determination of Five Phenolic Acids from Leaf of llex purpurea by HPLC
摘要
Abstract
AIM:To establish a HPLC method for simultaneous determination of five phenolic acid from leaves of Ilex purprea Hassk. METHOD: Chromatography was performed on a 5 μm HP-Hyspersil BDS-C1s column (250 mm x 4 mm). The mobile phase was acetonitrile-water(acidifled by 85% formic acid), and the five phenolic acids were eluted with a linear gradient program. DAD detector was used and signals were recorded at 260 nm and 330 nm. RESULT: Complete resolution of the five phenolic acids was obtained within 30 min. The method fulfills the requirements of precision, accuracy and stability. The recoveries of the five compounds were between 95% and 105%. CONGLUSION:This method is simple and effective, and can be used to determine phenolic acids from leaf of Ilex purprea Hassk.关键词
四季青/酚酸类成分/HPLC/DAD检测器/含量测定Key words
llex purprea Hassk./Phenolic acids/RP-HPLC/DAD detector/Determination of content分类
医药卫生引用本文复制引用
苗水,毕志明,李萍..HPLC测定四季青中5种酚酸类成分的含量[J].中国药科大学学报,2004,35(2):127-130,4.基金项目
国家杰出青年基金资助项目(No.33025046) （No.33025046）
