中国临床康复2003，Vol.7Issue(1)：128-129,2.
抑制脑血栓形成的硫代寡核苷酸的细胞摄取率和亚细胞分布
Cellular uptaking rate and subcellular distribution of thiooligonucleotide which can inhibit cerebral thrombosis
摘要
Abstract
AIM:To explore the membrane permeability, enzyme tolerance, and stability of phosphorothioate oligodeoxynucleotide(P ODN) which can inhibit cerebral thrombosis.METHODS:(1)Oligodeoxynucleotide was synthesized with phosphoramidite method in solid phase,3' end was modified with thiophosphoter.(2)Uptake and subcellular distribution of P ODN.P ODN was given directly.We observed total uptake rate of P ODN by ECV304 cells and subcellular distribution of P ODN.RESULTS:(1)ECV cells uptake more P ODN with increase of concentration,the uptake rate was about 10.8% - 11.9% .(2)Subcellular distribution of P ODN:P ODN was mainly distributed in nucleus especially nucleus sediments(69.3% - 77.2% )(P< 0.05).CONCLUSION:P ODN suppressing cerebral thrombosis has favorable transmembrane ability,enzyme resistance and stability.关键词
embdism and thrombosis/thionucleotides subcellular fractionsKey words
embdism and thrombosis/thionucleotides subcellular fractions分类
医药卫生引用本文复制引用
李黔宁,应大君,戴光明,郑健,王东武,肖道虹,刘勇,邓志宽,刘立杰,周永琴..抑制脑血栓形成的硫代寡核苷酸的细胞摄取率和亚细胞分布[J].中国临床康复,2003,7(1):128-129,2.基金项目
Supported by repressive effect of the triple helix forming oligodeoxynucleotide on expression of endothelial TF. Serial number: 39970269 （）
