中国病理生理杂志2001，Vol.17Issue(2)：128-130,3.
虎杖甙对心肌细胞钙的调节作用
The regulation of polydatin on
摘要
Abstract
AIM: To elucidate the mechanism of polydatin(PD) in increasingthe contractility of myocardial cells by observing the cytosolic free calcium concentration ([Ca2＋]i) of myocardial cells of rats. METHODS: The cells were labelled with fluo-3-AM, and [Ca2＋]i was determined by use of confocal microscopy (ACAS 570). RESULTS: In the study, we found that [Ca2＋]i of myocytes was elevated 10min after adding PD (0.6 mmol/L), but [Ca2＋]i of some cells increased to 111.80%±2.22% vs baseline, the others to 224.00%±24.33%. The effect of PD was inhibited remarkably by pretreated with EGTA(2mmol/L), verapamil (50 μmol/L), a kind of L-calcium channel antagnist, and tetrodotoxin ( 1 μmol/L), a kind of sodium channel blocker 10 min before PD, the fluorescence value were decreased to 53.00%±9.02% , 52.00%±7.07% and 72.67%±12.70% respectively vs baseline (P＜0.01). CONCLUSION: The results suggested that PD could increase [Ca2＋]i so as to enhance the contractility of myocardium, which might be related with the open of L-calcium channel and/or sodium channel.关键词
大鼠/心肌/钙分类
医药卫生引用本文复制引用
金春华,赵克森,刘杰,黄绪亮..虎杖甙对心肌细胞钙的调节作用[J].中国病理生理杂志,2001,17(2):128-130,3.基金项目
国家自然科学基金项目（No.39870936）；广东省博士启动基金资助 （）
