中国病理生理杂志2000，Vol.16Issue(12)：1292-1294,3.
NO在外源性高浓度Ca2+损伤心肌线粒体中的作用
Effects of nitric oxide on mitochondrial damage caused by exogenous calcium
梁晚益 1杨宗城 1黄跃生1
作者信息
- 1. 第三军医大学西南医院烧伤研究所，重庆 400038
- 折叠
摘要
Abstract
AIM: To study the effects of nitric oxide (NO) on mitochondrial damage caused by exogenous calcium. METHODS: Normal myocardial mitochondria were divided into three groups; L- arginine control group (CG), Ca2 + - damaged group (DG) and L - NAME - preserved group (PG). Mitochondria of all groups were incubated at 30℃ with reaction medium containing 20μmol/L EDTA, 100μmol/L CaC12 and 1 μmol/L L- NAME with 100μmol/L CaCl2 respectively. Then the NO2-/NO3- contents, mitochondrial viability and membrane potential were investigated. RESULTS: The NO2-/NO3 contents of DG was obviously higher than that of CG and PG, meanwhile, there was no obvious difference between CG and PG. Mitochondrial viability and membrane potential of DG were significantly lower than that of CG and PG, and negatively related to NO2-/NO3- contents ( r = - 0.5297, P < 0.01; r = -0.6041, P < 0.01 ). But, the mitochondrial viability and membrane potential of PG were still lower than that of CG. CONCLUSION: Exogenous calcium could activate mitochondrial nitric oxide synthase resulting in NO production and the latter play an important role in decreasing mitochondrial viability and membrane potential.关键词
线粒体/钙/一氧化氮Key words
Mitochondria/Calcium/Nitric oxide分类
生物科学引用本文复制引用
梁晚益,杨宗城,黄跃生..NO在外源性高浓度Ca2+损伤心肌线粒体中的作用[J].中国病理生理杂志,2000,16(12):1292-1294,3.
