国际眼科杂志2008，Vol.8Issue(7)：1293-1295,3.
脱细胞猪角膜基质体外支持角膜上皮和基质细胞的生长
Support of acellular porcine corneal stroma for growth of corneal epithelium and stromal cell in vitro
摘要
Abstract
AIM:To determine whether acellular porcine cornea stroma (APCS) could support the growth of the rabbit corneal cells in vitro.METHODS: APCS was prepared. The rabbit's corneal epithelium and stromal cells were cultured and seeded on, APCS in vitro.The observation of phase contrast photograph and histological examination were performed.RESULTS: Histological examination showed the epithe- lium grew on the scaffold of APCS in 2-3 layers at 10th day. The stromal cells adhered to the surface of the scaffold after 24 hours and invaded into the interlaminar of the material at 5th day.CONCLUSION: These results indicate that APCS can support the growth and proliferation of the corneal epithelium and stromal cells in vitro.关键词
脱细胞猪角膜基质/角膜上皮/角膜基质细胞/组织工程Key words
acellular porcine cornea stroma/ cornealepithelium/ corneal stromal cells/ tissue engineering分类
医药卫生引用本文复制引用
林旭初,惠延年,孟浩,张永杰,金岩..脱细胞猪角膜基质体外支持角膜上皮和基质细胞的生长[J].国际眼科杂志,2008,8(7):1293-1295,3.基金项目
本研究受"863"高新技术发展基金资助(No.2005AA205241) （No.2005AA205241）
This study was supported by theDevelopment of High and New Science and Technology (863Project) of China ( No. 2005AA205241 ) （863Project）
