国际眼科杂志2008，Vol.8Issue(7)：1299-1303,5.
AG对大鼠慢性高眼压视网膜Caspase-9表达的影响
Expression of caspase-9 affected by AG on retina of rats with chronic IOP elevation
聂庆珠 1沙倩 1王英爽 1归东梅 1刘致力 1高殿文1
作者信息
- 1. 110004,中国辽宁省沈阳市,中国医科大学附属盛京医院眼科
- 折叠
摘要
Abstract
AIM: To study caspase-9 expression on rat retina in the process of chronic elevation of IOP and the changes with the application of amino guanidine (AG), thus to investigate potential protective function of AG to rat retina with chronic elevation of IOP.METHODS: Immunohistochemistry, RT-PCR and Western blot were used to observe retinal morphology and expression of caspase-9 at different time points of rat with chronic IOP elevation, both affected or not affected by the application of AG.RESULTS: Compared with control group, as time passed retina of experimental group gradually had detectable morphological changes. On 21st day of chronic IOP elevation, retinas became thinner and the quantity of retinal ganglion cells (RGCs) decreased; caspase-9 expression increased, consistent with the morphological changes. The group using AG presented relatively smaller morphology changes and less expression of caspase-9.CONCLUSION: Apoptosis-related gene caspase-9 played a part in the process of chronic IOP elevation; AG protects retina by down-regulating expression of caspase-9.关键词
视网膜/慢性高眼压/凋亡/氨基胍(AG)/半胱天冬酶-9(Caspase-9)Key words
retina/ chronic IOP elevation/ apoptosis/amino guanidine/ caspase-9分类
医药卫生引用本文复制引用
聂庆珠,沙倩,王英爽,归东梅,刘致力,高殿文..AG对大鼠慢性高眼压视网膜Caspase-9表达的影响[J].国际眼科杂志,2008,8(7):1299-1303,5.
