中国现代医学杂志2004，Vol.14Issue(7)：1-3,7,4.
EGR1在调控肝再生过程中的功能研究
Functional importance of EGR-1 in regulating liver regeneration
廖允军 1张阳德 2王成友 1David Rudnick3
作者信息
- 1. 深圳市第二人民医院普外科,深圳,518035
- 2. 卫生部肝胆肠外科研究中心,湖南,长沙,410008
- 3. 华盛顿大学医学院附属SAINT LOUIS 儿童医院,美国 SAINT LOUIS 63011
- 折叠
摘要
Abstract
Objective: To investigate the functional importance of EGR-1 gene in regulating liver regeneration. Methods: EGR-1 mRNA levels were quantified in quiescent and regenerating liver derived from replicate wildtype mice at serial time-points after partial hepatectomy or sham surgery by using real time RTPCR. EGR-1 protein in whole cell lysate derived from wildtype and EGR-1 null mouse liver at 48 and 72hours after partial hepatectomy were compared and menitored by Western blot. Results: The data showed that during liver regeneration induction of EGR-1 gene expression occur 6～12 hours after partial hepatectomy,then declines, and increases again at 48 hours after partial hepatectomy, and that EGR-1 protein expression was detectable 12～48 hours after surgery. Thus the EGR-1 protein was present in regenerating liver at the time corresponding to hepatocellular mitotic progression. Conclusions: EGR-1 is indeed necessary for normal liver regeneration to proceed efficiently. The functional role of EGR-1 during liver regeneration involves modulation of mitotic cell cycle progression.关键词
Egr-1/肝再生/细胞周期Key words
EGR-1/liver regeneration/cell cycle分类
医药卫生引用本文复制引用
廖允军,张阳德,王成友,David Rudnick..EGR1在调控肝再生过程中的功能研究[J].中国现代医学杂志,2004,14(7):1-3,7,4.
