中华医学杂志（英文版）2009，Vol.122Issue(11)：1300-1304,5.DOI:10.3760/cma.j.issn.0366-6999.2009.11.012
Comparison of hyaluronidase expression, invasiveness and tubule formation promotion in ER (-) and ER (+) breast cancer cell lines in vitro
Comparison of hyaluronidase expression, invasiveness and tubule formation promotion in ER (-) and ER (+) breast cancer cell lines in vitro
WANG Xiao-yi 1TAN Jin-xiang 1Marc Vasse 2Bertrand Delpech 2REN Guo-sheng3
作者信息
- 1. Department of Endocrine Surgery, First Affiliated Hospital,Chongqing Medical University, Chongqing 400016, China
- 2. Groupe MERCI, Faculte de M&tecine-Pharmacie, 22 boulevard Gambetta, 76183 Rouen cedex, France
- 3. Laboratoire d'Oncoiogie Moleculaire, Centre Henri-Becquerel, rue d'Amiens, 76000 Rouen, France
- 折叠
摘要
Abstract
Background Hyaluronidase (Hyase) is an enzyme which hydrolyses hyaluronan (HA), a large nonsulfated glycosaminoglycan. Several genes have been identified to code for hyaluronidases in humans. Its role has only recently been underlined in the invasion of prostate cancer, colonic cancer, and breast cancer. Moreover, the findings were in agreement with some experimental results which showed that HA-derived oligosaccharides had angiogenesis-promoting activity. All these findings prompted us to investigate factors that had been characterized as putative invasive factors in different human breast cancer-derived cell lines.Methods We selected two series of human breast cancer-derived cell lines whose expression of estrogen receptors (ER) was previously published. Hyaluronidase secretion in culture medium and expression of matrix metallo-proteinase (MMP)-9, cathepsin-D (cath-D) and vascular endothelial growth factor (VEGF) by cells were determined. We also investigated cell invasiveness in the Matrigel invasion assay, and studied the capability of cancer cells to promote in vitro formation of tubules by endothelial cells.Results ER(-) cells secreted significantly more hyaluronidase (P <0.001) and expressed significantly more VEGF (P <0.01), MMP-9 (P <0.05) and cath-D (P <0.0001) than ER(+) cells. Invasion through Matdgel by ER(-) Hyase(+) cells was significantly higher than that by ER(+) Hyase(-) cells (P<0.05). In both cases, invasion was decreased by heparin (P <0.05). When ECV-304 endothelial cells were co-cultivated in millicell chambers with cancer cells, ECV-304 cells were induced to form tubules. Tubule formation was demonstrated to be more prominent with ER(-) Hyase(+) cells than with ER(+) Hyase(-) cells (P <0.05).Conclusion Invasive features of ER(-) breast cancer cells can be characterized in vitro by an invasive Matrigel assay,as the induction of tubule formation by ECV-304 endothelial cells, higher secretion of hyaluronidase, and higher expression of proteinases MMP-9, cath-D, and the angiogenesis promoting factor VEGF.关键词
angiogenesis/ breast neoplasms/ receptors, estrogen/ hyaluronidase/ invasion/ peptide hydrolasesKey words
angiogenesis/ breast neoplasms/ receptors, estrogen/ hyaluronidase/ invasion/ peptide hydrolases分类
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WANG Xiao-yi,TAN Jin-xiang,Marc Vasse,Bertrand Delpech,REN Guo-sheng..Comparison of hyaluronidase expression, invasiveness and tubule formation promotion in ER (-) and ER (+) breast cancer cell lines in vitro[J].中华医学杂志（英文版）,2009,122(11):1300-1304,5.
