传感技术学报2008，Vol.21Issue(8)：1301-1306,6.
基于纳米金/硫堇修饰金电极的脱落酸安培免疫传感器的研制
Research of Amperometric Immunosensor Based on Gold Nanoparticle/Thionine Modified Gold Electrode for the Detection of Abscisic Acid
摘要
Abstract
An amperometric immunosensor based on gold nanoparticle /thionine modified gold electrode for the determination of phytohormone abscisic acid (ABA) was proposed. The sensor was constructed based on the catalytic wave system of H2O2 and HRP with thionine as the medium. In the presence of HRP, the reduction current of thionine was substantially increased by the addition of H2O2 due to the catalytic wave system, and the current increase was dependent upon the activity of HRP. The activity of HRP could be modulated by the binding event of ABA to ABA-antibody conjugated HRP, producing a decrease in the catalytic wave. Bovine serum albumin (BSA) was employed to block the possible remaining active sites of the thionine monolayer to avoid the non-specific adsorption. Assay conditions were optimized including the proportion of ABA-antibody conjugated HRP and thionine, the incubation time, the pH of working buffer and the concentration of the H2O2. The reduction current of the immunosensor decreases linearly in the range of ABA from 0.5 ng/mL to 1 000 ng/mL, while the linear regression equation was y=0.020 9x+17.071. The correlation coefficient was 0.992 2. The detection limit was 0.2 ng/mL.关键词
培免疫传感器/ABA/纳米金/硫堇/金电极Key words
amperometric immunosensor/abscisic acid/gold nanoparticle/thionine/gold electrodeEEACC:7230J分类
信息技术与安全科学引用本文复制引用
李巍,黄志刚,李合松,钟菲菲,萧浪涛,沈国励..基于纳米金/硫堇修饰金电极的脱落酸安培免疫传感器的研制[J].传感技术学报,2008,21(8):1301-1306,6.基金项目
植物激素的高灵敏免疫传感测定仪研制(30670190 c01020305) （30670190 c01020305）
国家质监总局质检公益科研专项资助:农产品及其制品中生长调节剂标准研究项目资助(2007GYB146) （2007GYB146）
