青岛大学医学院学报2003，Vol.39Issue(2)：130-132,136,4.
扇贝多肽对紫外线损伤Hela细胞的保护作用
PROTECTIVE EFFECTS OF POLYPEPTIDES FROM CHLAMYS FARRERI AGAINST ULTRAVIOLET-INDUCED DAMAGE ON Hela CELLS
刘晓萍 1于业军 1王玉贞 1房丽华 1王春波1
作者信息
- 1. 青岛大学医学院组织学与胚胎学教研室,山东,青岛,266021
- 折叠
摘要
Abstract
Objective The investigate the effect of polypeptide isolated from Chlamys Farreri (PCF) on Hela cells damaged by ultraviolet(UV). Methods The suspension of Hela epithelia cells (5×108/mL ) cultured for 24 hours were prepared and 1 mL of it was put into each hole of 24-hole plates. Some Hela cells were randomly divided into six groups: control group (C), modal group(M), 5 g/L PCF group, 10 g/L PCF group, 20 g/L PCF group and 10 g/L Vitamin-C (Vc) group. After incubation with RPMI-1640, PCF, Vitamin C, respectively, for 10 minutes, the cells were exposed to UVA ( irradiation intensity: 3 650 μJ/cm2), with the exception of the mice in C group. Some other Hela cells were divided into another six groups, they were treated almost the same as above, but irradiated by UVB ( irradiation intensity: 7.15×10-5 J/cm2). The intracellular free calcium (Ca2+), apoptosis and cell death rate were measured by flow cytometry (FCM). Enzyme activities and malondialdehyde (MDA ) were determined by biochemical assays. Results The apoptosis and cell death rate were decreased and the contents of intracellular Ca2+ and activities of succinate dehydrogenase (SDH) as well as the activities of GSH-px, SOD,CAT increased and the MDA decreased by the action of PCF. Conclusion It is suggested that PCF could protect the cells from the damage caused by UVA and UVB by enhancing the activities of antioxidant enzymes.关键词
扇贝多肽/紫外线/凋亡/Hela细胞Key words
polypeptide from Chlamys Farreri/ultravioletrags/apoptosis/Hela cells分类
医药卫生引用本文复制引用
刘晓萍,于业军,王玉贞,房丽华,王春波..扇贝多肽对紫外线损伤Hela细胞的保护作用[J].青岛大学医学院学报,2003,39(2):130-132,136,4.
