中华医学杂志（英文版）2001，Vol.114Issue(12)：1313-1316,4.
IgA肾病和紫癜性肾炎患者单核细胞白细胞介素－1受体拮抗剂的产生能力受其基因多态性的影响
Gene polymorphism in IL-1 receptor antagonist affects its production by monocytes in IgA nephropathy and Henoch-Schonlein nephritis
刘志红 1杨俊伟 1陈朝红 1龚如军 1黎磊石1
作者信息
- 1. 南京大学医学院临床学院肾脏病研究所,南京,210002
- 折叠
摘要
Abstract
Objective To define the functional significance of IL-1 receptor antagonist (IL-1ra) gene polymorphism and to investigate, the production of IL-1ra by monocytes from individuals with different genotypes of IL-1.Methods The genotype of IL-1ra was detected by polymerase chain reaction (PCR). Peripheral monocytes obtained from patients with immunoglobin A nephropathy (IgAN), Henoch-Schonlein purpura nephritis (HSPN) and normal subjects were matched in sex and age between the IL1RN-2 allele carriers and non-carriers. The secretion of IL-1ra, IL-1α and IL-1β in the supernatant of GM-CSF (10ng/ml) treated and untreated monocytes were measured by ELISA.Results The secretion of IL-1ra by monocytes stimulated with GM-CSF was significantly higher in the IL1RN-2 allele non-carriers than those of carriers both in IgAN (21.55±3.08 vs 13.85±2.24ng/ml, P<0.001) and HSPN (23.72±6.68 vs 12.67±2.24ng/ml, P<0.01) as well as in normal controls (20.29±1.45 vs 10.51±2.3ng/ml, P<0.001). All showed no significant differences in monocyte secretion of IL-1α and IL-1β by GM-CSF stimulation between the IL1RN-2 allele carriers and non-carriers. Conclusions These results indicate that a functional correlation of the IL1RN-2 allele and IL-1ra production is present in patients with IgAN and HSPN. This gene polymorphism control of IL-1ra production may contribute to the variety of clinical responses to inflammatory stimulation in individuals with different genotype of IL-1ra.关键词
IL－1受体拮抗剂 基因多态性 IgA肾病 紫癜性肾炎Key words
IL-1 receptor antagonist/gene polymorphism/IgA nephropathy/Henoch-Schonlein nephritis分类
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刘志红,杨俊伟,陈朝红,龚如军,黎磊石..IgA肾病和紫癜性肾炎患者单核细胞白细胞介素－1受体拮抗剂的产生能力受其基因多态性的影响[J].中华医学杂志（英文版）,2001,114(12):1313-1316,4.
