华东理工大学学报（自然科学版）2001，Vol.27Issue(2)：131-314,184.
φ10启动子控制下大肠杆菌中rhNTA表达的乳糖诱导
The Lactose Induction for the Expression of rhNTA under the Control of φ10 Promoter in Escherichia coli
摘要
Abstract
The process of expression of rhNTA (a new thromobolytic agent) gene using lactose as an inducer under the control of φ10 promoter in Escherichia coli is investigated. The recombinant protein rhNTA is 40%～50% of the total cell protein and the wet cell weight is 86.0～100.2 g/L of the culture broth after 12 h cultivation. It is manifested that the key components of the subtract and the strategy of lactose induction play an important role with regard to expression of recombinant protein. The percentage of yeast extract of the nitrogen source during the growth phase and in the basal medium during the phase of induction and the time and condition that the induction starts are effective. The target protein in the cell of Escherichia coli was mainly occupied by soluble part of rhNTA protein.关键词
大肠杆菌/φ10启动子/诱导/乳糖/表达分类
生物科学引用本文复制引用
陆兵,夏杰,徐殿胜,陈国豪..φ10启动子控制下大肠杆菌中rhNTA表达的乳糖诱导[J].华东理工大学学报（自然科学版）,2001,27(2):131-314,184.
