首页|期刊导航|中国农业科学(英文版)|In Vitro Selection and Identification of Drought-Tolerant Mutants in Sweetpotato
中国农业科学(英文版)2003，Vol.2Issue(12)：1314-1320,7.
In Vitro Selection and Identification of Drought-Tolerant Mutants in Sweetpotato
In Vitro Selection and Identification of Drought-Tolerant Mutants in Sweetpotato
摘要
Abstract
In vitro selection of drought-tolerant mutants in sweetpotato cv. Lizixiang was studied by using PEG6000 as selection stress. Embryogenic suspension cultures were cultured in MS medium containing 0-35% PEG6000 and 2 mg L-1 2, 4-dichlorophenoxyacetic acid (2, 4-D). The results indicated that 30%PEG6000 can be used for the optimal selection stress of drought-tolerance. Embryogenic suspension cultures irradiated with 80 Gy gamma-ray were cultured in MS medium containing 30 % PEG6000 and 2 mg L-1 2,4-D and 20 drought-tolerant cell aggregates were obtained. These cell aggregates were transferred to solid MS medium supplemented with 2 mg L-1 2,4-D and formed embryogenic callus with somatic embryos. The embryogenic callus with somatic embryos was further transferred to MS medium supplemented with 1 mg L-1 abscisic acid (ABA), resulting in the germination of somatic embryos. In this study a total of 18 regenerated plants were obtained. The regenerated plants were transplanted in a greenhouse and 11 lines were formed. The analysis on drought treatment of seedlings, water retaining capacity of leaves and coefficient of drought-tolerance showed that 3 lines had significant drought-tolerance in comparison with the control plants.关键词
Sweetpotato/Drought-tolerant mutants/In vitro selection/IdentificationKey words
Sweetpotato/Drought-tolerant mutants/In vitro selection/Identification分类
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WANG Yu-ping ,LIU Qing-chang ,LI Ai-xian ,ZHAI Hong,ZHANG Song-shu,LIU Bao-li..In Vitro Selection and Identification of Drought-Tolerant Mutants in Sweetpotato[J].中国农业科学(英文版),2003,2(12):1314-1320,7.基金项目
This research was supported by Outstanding Younger Science Foundation of China (30225028),the National High Tech R&D Program, China(863 Program, 2002AA241031), the National Science Technology Program ( 2001BA511B ) and the Teaching and Research Award Pro （30225028）
