色谱2001，Vol.19Issue(1)：13-15,3.
高效液相色谱法测定米根霉乳酸发酵液中乳酸的光学纯度
Determination of Enantiomeric Purity for Lactic Acid in Fermentation Broth by Rhizopus Oryzae with High Performance Liquid Chromatography
摘要
Abstract
A procedure for the resolution of DL-lactic acid and th edeterminatio n of D-isomer ratio in L-lactic acid fermentation broth by Rhizopus oryzae is desc ribed. The effects of pH of mobile phase and concentration of chiral mobile pha s e additives on resolution of DL-lactic acid were investigated. The optical is om ers of lactic acid were resolved by RP-HPLC with 2,3,6-tri-O-β-cyc lode xtrin(TM-β-CD) as a chiral mobile phase additive, and C18 column as stationary phase, and detected at wavelength 210 nm. The results sh owed that a correction factor should be introduced into the equation for ca lc ulation of the percentage of D-lactic acid, because the UV absorption of D-lac tic acid and L-lactic acid might not be the same when TM-β-CD was presen t. Quantitation was achieved with external standard method, the average rec overy was 100.4%, and the relative standard deviation was 0.82%. This method can be used for the determination of the percentage of D-isomer in L-lactic a cid fermentation broth by Rhizopus oryzae, and it is simple, rapid and accur ate. T he results showed that the mass fraction of D-isomer in the fermented broth inc reased during the period of storage.关键词
高效液相色谱法/乳酸/手性分离分类
化学化工引用本文复制引用
白冬梅,赵学明,胡宗定..高效液相色谱法测定米根霉乳酸发酵液中乳酸的光学纯度[J].色谱,2001,19(1):13-15,3.基金项目
国家自然科学基金资助项目( （）
