军事医学科学院院刊2001，Vol.25Issue(1)：13-16,4.
HLA-DR分子的分离纯化
Purification of HLA-DR molecules
贾帅争 1孙红琰 1刘晓达 1杜芝燕 1王全立1
作者信息
摘要
Abstract
Objective:To purify HLA-DR molecules. Methods: Anti-HLA-DR antibody L243 was purified and coupled with CNBr activated Sepharose 4B gel. Immunoaffinity column was used to purify HLA-DR molecules. Results:Twenty micrograms of HLA-DR molecules were isolated from about 5 g Epstein-Barr virus-transformed human B lymphoblastoid cell line RAJI lysates by affinity chromatography. The purified HLA-DR molecules existed in α/β heterodimers form and could bind to conformation-dependent antibody L243. These HLA-DR molecules were separated into two strands,α and β,by boiling denaturation. These results are the basis for studying MHC Ⅱ binding peptide motif and CD4＋ T cell epitopes of antigens in future.关键词
组织相容性抗原Ⅱ类/HLA-DR抗原/免疫亲和纯化/色谱法分类
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贾帅争,孙红琰,刘晓达,杜芝燕,王全立..HLA-DR分子的分离纯化[J].军事医学科学院院刊,2001,25(1):13-16,4.
