北京大学学报（医学版）2001，Vol.33Issue(2)：132-135,4.
乙型肝炎病毒相关性肾炎肾组织中HBV DNA的原位检测
Detection of HBV DNA in kidneys of HBV-associated glomerulonephritis using in situ hybridization and in situ PCR technique
王素霞 1邹万忠 2张波1
作者信息
- 1. 北京大学基础医学院病理学系，北京 100083
- 2. 北京大学基础医学院病理学系,
- 折叠
摘要
Abstract
Objective: To detect the localization of HBV DNA in kidneys of HBV-associated glomerulonephritis, and investigate the pathogenesis of HBV-associated glomerulonephritis. Methods: 45 cases of renal biopsy specimens (38 cases had glomerular deposition of HBV antigens, 7 cases were negative for serologic and histologic HBV antigens) were examined for HBV DNA by in situ hybridization (ISH) and in situ PCR (IS-PCR). Results: The positive rate of HBV DNA in renal biopsy was 71% (27/38) in patients with glomerular deposition of HBV antigens. HBV DNA was found in 19 cases (19/26, 73%) of HBV-associated glomerulonephritis; and in 8 cases (8/12, 67%) of IgA nephropathy and lupus nephritis with glomerular deposition of HBV antigens. HBV DNA was detected mainly in the cytoplasm of tubular epithelia with ISH; IS-PCR showed that HBV DNA was localized not only in cytoplasm of tubular epithelia, but also in nuclei of tubular epithelia, in nuclei and cytoplasm of glomerular epithelia and mesangial cells, and in GBM. Conclusion: Our study suggested the presence of HBV infection and replication in glomerular cells and renal tubular epithelia, indicating an etiologic role of HBV in HBV-associated glomerulonephritis.关键词
肾炎/微生物学/肝炎病毒,乙型/分离和提纯/肾/微生物学/DNA/分析分类
医药卫生引用本文复制引用
王素霞,邹万忠,张波..乙型肝炎病毒相关性肾炎肾组织中HBV DNA的原位检测[J].北京大学学报（医学版）,2001,33(2):132-135,4.
