中华医学杂志（英文版）2003，Vol.116Issue(9)：1329-1332,4.
Identification of genes expressed during myocardial development
Identification of genes expressed during myocardial development
摘要
Abstract
Objective To identify genes expressed in the fetal heart that are potentially important for myocardial development and cardiomyocyte proliferation.Methods mRNAs from fetal (29 weeks) and adult cardiomyocytes were use for suppression subtractive hybridization (SSH). Both forward (fetal as tester) and reverse (adult as driver) subtractions were performed. Clones confirmed by dot-blot analysis to be differentially expressed were sequenced and analyzed.Results Differential expressions were detected for 39 out of 96 (41%) clones on forward subtraction and 24 out of 80 (30%) clones on reverse. For fetal dominating genes, 28 clones matched to 10 known genes (COL1A2, COL3A1, endomucin, HBG1, HBG2, PCBP2, LOC51144, TGFBI, vinculin and PND), 9 clones to 5 cDNAs of unknown functions (accession AK021715, AF085867, AB040948, AB051460 and AB051512) and 2 clones had homology to hEST sequences. For the reverse subtraction, all clones showed homology to mitochondrial transcripts.Conclusions We successfully applied SSH to detect those genes differentially expressed in fetal cardiac myocytes, some of which have not been shown relative to myocardial development.关键词
cardiomyocyte/development/gene expressionKey words
cardiomyocyte/development/gene expression分类
医药卫生引用本文复制引用
陈小圆,陈健宏,张碧琪,梁瑛,梁平..Identification of genes expressed during myocardial development[J].中华医学杂志（英文版）,2003,116(9):1329-1332,4.基金项目
This study was supported by a grant of CRCG from the University of Hong Kong. （）
