摘要
Abstract
BACKGROUND:Because of the complex pathological mechanism of ischemic stroke,the research on cocktail plan is prospective probably. OBJECTIVE:To study the protection of compound ferulic acid against cerebral ischemic reperfusion injury in experimental rats and to explore its function mechanism. DESIGN:A completely randomized controlled trial. SETTING and MATERIALS:The trial was carried out in the Second Clinical College Affiliated to Chongqing Medical University.The healthy adult Wistar rats were provided by the Experimental Animal Center of Chongqing Medical University. INTERVENTION:By blocking the bilateral common carotid arteries, bleeding on the tails and repeating ischemic reperfusion,the cerebral ischemia models of rats were established.The gastric perfusion was applied with physiological saline,danshen medical liquid or compound ferulic acid medical liquid for 5 days successively. MAIN OUTCOME MEASURES:Observation of expression and histopathology on endothelin-1(ET-1) of the cerebral tissue of rats,calcitonin gene-related peptide(CGRP),malonaldehyde, energetic metabolism and heat shock protein 70(HSP70). RESULTS:In the model group, the contents of ET-1 and malonic aldehyde were the maximum,the content of CGRP was the minimum and the energetic metabolism was disturbed.In high dosage group of compound ferulic acid, the contents of ET-1[(0.73± 0.04) ng/g] of cerebral tissue, lactic acid[(16.28± 1.1) μ mol/g]and malonic aldehyde[(116.8± 7.55) nmol/g] were remarkably reduced,and the contents of ATP[(18.22± 0.83) μ g/g] and CGRP[(1.62± 0.13) ng/g],and Na+-K+-ATP enzymatic activities [(747.82± 63.94) nkatal]were significantly increased,which indicated significant difference(P< 0.05).The quantity-effect relation was concerned to the above-mentioned functions of compound ferulic acid,for the effect in the high dosage group was superior to that in the positive control group with danshen.In compound ferulic acid treatment group, the expression of HSP70 in cerebral cortex was remarkably improved and the severity of cerebral ischemic injury reduced. CONCLUSION:Compound ferulic acid acted on the protection against cerebral ischemic reperfusion injury,the mechanism of which was related to the energetic metabolic improvement of cerebral cells,antagonistic free radical,microcirculation improvement,etc.关键词
再灌注损伤/功能恢复/大鼠/药物疗法分类
医药卫生
