第一军医大学学报2005，Vol.25Issue(11)：1341-1343,1356,4.
KBV200细胞多药耐药与蛋白激酶C表达上调的关系
Correlation of multidrug resistance with up-regulation of protein kinase C expression in KBV200 cells
摘要
Abstract
Objective To investigate protein kinase C (PKC) expression and its association with multidrug resistance (MDR) in KBV200 cells. Methods KBV200 cells were preincubated with PKC activator phorbol-12-myristate-13-acetate (PMA, 200nmol/L) and PKC activity was assayed by measurement of peptide substrate 32p incorporation from [γ-32p]ATP, with the cells without PMA preincubation serving as the control. Western blotting was performed for assessing the expression of PKC isoform, and the cell inhibition rate was evaluated by MTT assay. Results PMA preincubation of the cells significantly enhanced the activity of the total PKC and the membrane fraction, but lowered the PKC activity of the cytosol fraction, as compared with the cells without PMA treatment (P＜0.01). PKCα expression was upregulated in the membrane fraction and down-regulated in the cytosol fraction in KBV200 cells after PMA preincubation. PKCβ expression was slightly elevated in the cytosol fraction but exhibited no obvious changes in the membrane fraction after PMA pretreatment of the cells. The values of IC50 of vincristine and adriamycin in PMA-treated cells were increased to 2275.5 nmol/L and 233.25 nmol/L, respectively (P＜0.01).Conclusion PMA can increase the multidrug resistance of KBV200 cells, which suggests the possible involvement of PKC in the mechanism ofmultidrug resistance of tumor cells.关键词
蛋白激酶C/多药耐药/长春新碱/阿霉素/佛波酯Key words
protein kinase C/multidrug resistance/vincristine/adriamycin/phorbol-12-myristate-13-acetate分类
医药卫生引用本文复制引用
袁亚维,孙爱民,李传刚..KBV200细胞多药耐药与蛋白激酶C表达上调的关系[J].第一军医大学学报,2005,25(11):1341-1343,1356,4.基金项目
Supported by National Natural Science Foundation of China (39870809) （39870809）
